Methods An EOSA kit was prepared using the further purified tetanus toxoid as coated antigen, and HRP-labeled sheep anti-human IgG as antibody, and the sensitivity, specificity and reproducibility of the prepared kit were studied.

• 方法 以精制破伤风类毒素进一步纯化后作为包被抗原,辣根过氧化物酶(HRP)标记羊抗人igg作为抗体制备ELISA试剂盒,并进行敏感性、特异性及重复性检测。