Methods Amplify Rpf E gene from the genomic DNA of M.tuberculosis H37Rv by PCR,insert into expression vector pET32a(+) and transform to E.

• 方法采用PCR方法从结核分枝杆菌H37Rv基因组DNA中扩增出RpfE基因,双内切酶消化后,与同样酶消化的pET32a(+)载体连接,转化大肠杆菌Top10。