Methods AFA were detected by immunoblotting (IB) in 860 serum samples including 388 from RA (172 cases with early RA and 216 cases with late RA), 422 from non-RA rheumatic diseases and 50 cases of healthy blood donors.

• 方法对860例研究对象,包括388例RA患者(其中早期172例,中晚期216例),422例非RA的风湿性疾病患者,50名健康对照,用免疫印迹法(IB)检测血清中的AFA。