Method:Stimulated the endothelial cells with aqueous areca nut extract(AANE) and the cultured medium was aspirated.Then the level of NO produced by endothelial cells was detected by method of ELISA.

• 方法:用槟榔提取物干预体外培养的内皮细胞,收集培养上清,用ELISA方法检测内皮细胞分泌一氧化氮(NO)的浓度。