Method: The collagen protein-heparan sulfate scaffold was made by frozen-lyophilized.Hippocampus-derived NSCs of neonatal mouse were cultured and combined with scaffold biomaterials in vitro.

• 方法： 以冷冻干燥法制备胶原蛋白-硫酸肝素支架，体外培养新生小鼠海马神经干细胞。