Method Peripheral blood of 98 neonats HIE AND 63 normal neonates were collected. The active level of NO were determined by RIA and colorimetry. The levels of IL-6 and IL-8 were determined by enzyme-linked immunosorbent assay(ELISE).

• 方法采用分光光度计比色法和酶标免疫吸附法分别测定98例HIE患儿及63例正常新生儿NO、IL-6、IL-8水平。