Mediated by PMS,the NADH resulted from the reaction catalysed by LDH isozymes can reduce K 3Fe(CN) 6 into K 4Fe(CN) 6 . Then K 4Fe(CN) 6 can react with FeCl 3 resulting in Prussian blue which dyes LDH isozymes.
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- 以N 甲基吩嗪甲基硫酸盐 (PMS)为介体 ；使乳酸脱氢酶 (LDH)同工酶催化反应产生的NADH将铁氰化钾还原为亚铁氰化钾 ；利用亚铁氰化钾与三氯化铁反应生成普鲁士蓝的性质 ；实现对乳酸脱氢酶同工酶进行染色的目的 .
