Measure of BMD: using HOLOGIC double-function X-ray bone intensity determinator, the BMD of lumber 3 and right femur shaft were examined at pre-ovariectomy, 12 weeks post-ovariectomy, 9 weeks after drug treatment.

• 活体下使用双能X线骨密度仪测定去卵巢前、去卵巢后12周第4,第5腰椎椎体以及右侧股骨骨折处骨密度(BMD)值; 药物治疗后9周,活体下使用骨密度仪测定第4,第5腰椎椎体BMD值,处死大鼠后完整取出右股骨,取出克氏针,测定右侧股骨骨折处BMD值。