McAb 3A_5, identified as IgGl, was directed against human hepatoma BEL--7402 cells. By ELISA, McAb 3A_5 and the Fab fragment were strongly reactive with hepatoma cells and weakly reactive with KB cells.

• 单抗3A_5和Fab片段分别与抗癌抗生素C1027偶联,偶联物经克隆生成法测定对肝癌细胞有很强的杀伤作用,C1027,Fab-C1027和3;