METHODS:RT-PCR analysis was used to detect the expression level of KiSS-1 mRNA in human pancreatic cancer cell line AsPC-1 and tissues of 40 cases of pancreatic cancer, 27 cases of their metastastic site and 9 cases of normal pancreatic tissues.

• 方法:应用RT-PCR方法检测KiSS-1基因mRNA在人胰腺癌细胞株AsPC-1、40例胰腺癌及27例转移灶和9例正常胰腺组织中的表达情况。