METHODS: The separation of D-NNA and L-NNA in plasma was achieved by chiral ligand exchange method via CEC. An aqueous eluent containing aspartame (2 mmol/L) sufficiently mixed with Cu2+ was used.
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美
- 方法采用CEC手性配体交换模式检测血浆样品中D-硝基精氨酸(D-NNA)和L-硝基精氨酸(L-NNA);流动相为50mmol/L醋酸缓冲液[pH5.;含2mmol/Laspar-tame;1mmol/LCu2+和5%25(v/v)甲醇];;