METHODS The orthogonal design was taken for the optinization,the content of 5-HMF was determined by RP-HPLC and the content of total saccharide was measured with the phenol-sulfuric acid method.

• 目的:以5-羟甲基糠醛及熟地黄总糖含量为评价指标,优选熟地黄最佳提取工艺。