METHODS Portal vein cannulation and perfusion combined with collagenase digestion were used to isolate pig pup hepatocytes.They were then cultured and passaged into the conditioned medium.

 
  • 方法 用门静脉插管原位灌注结合胶原酶消化法分离乳猪肝细胞;应用条件培养基对其进行体外培养;观察其形态、增殖、分化和功能等方面的变化.
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