METHODS PCR, Chinese ink smear and fungal culture were used respectively in the detection of cryptococcus neoformans in cerebrospinal fluid of 50patients.

• 方法 应用聚合酶链反应检测 5 0例临床脑脊液标本中的新型隐球菌 ；同时用直接涂片墨汁复染、真菌培养进行对比检测 .