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- Methods DNA of Leptospira interrogans in patient s sera was detected by using PCR and antibody by MAT. 方法应用PCR技术,测定患者血清钩体DNA和MAT法测血清中钩体抗体。
- Objective To clone the flagellar biosynthesis genes flhA,flhB_2 and fliR of Leptospira interrogans and construct their prokaryotic expression system. 目的克隆问号钩端螺旋体鞭毛相关基因flhA、flhB2和fliR,并构建其原核表达载体。
- This strain was identified as serogroup Manhao of L. interrogans, and it was different with the 5 known serovars in this serogroup. 方法采用经典的血清学分类检定法和脉冲场凝胶电泳分析法。
- Objective To study the immunoprotective property of the 39kD hydrophobic outer membrane protein of Leptospira interrogans (OmpL39) to guinea pigs. 目的女人研究钩体蛋白抗原的免疫保护力及其机制。
- The rat lung and kidney were taken to examine the epidemic hemorrhagic fever antigen, isolate hantaan virus, and culture Leptospira interrogans. 取鼠肺和鼠肾分别检验流行性出血热抗原及分离病毒和分离培养钩端螺旋体。
- Objective To develop a polymerase chain reaction (PCR) for specifically detecting all serogroups of leptospira interrogans epidemic in China. 目的建立一种能特异检测我国致病性钩端螺旋体所有血清型的PCR方法。
- There are more than 100 ORFs encoding outer membrane proteins sequences of L. interrogans serogroup Icterohaemorrhagiae serovar Lai predicted (http://www.chgc.sh.cn/lep/). 随着钩体黄疸出血群赖型赖株全基因组序列测序的完成和释放(http://www.;chgc
- To our viewpoint,this method could be applied to rapidly identify and preliminary classify Leptospira interrogans and one of effective molecular epidemiological means. 结论：用此法对钩端螺旋体进行快速鉴定，初步分类，不失为分子流行病学调查的一种有效手段。
- Leptospria Icterohaemorrhagiae Antiserum 黄疸出血型钩端螺旋体抗血清[生物制剂]
- Leptospira interrogans serogroup australis 澳大利亚问号血清型钩端螺旋体
- Leptospira interrogans serogroup autumnalis 秋季热问号血清型钩端螺旋体
- Leptospira interrogans serogroup bataviae 巴塔维亚问号血清型钩端螺旋体
- Leptospira interrogans serogroup canicola 犬型问号血清型钩端螺旋体
- Leptospira interrogans serogroup grippotyphosa 感冒伤寒型问号血清型钩端螺旋体
- Leptospira interrogans serogroup hebdomidis 七日热问号血清型钩端螺旋体
- Leptospira interrogans serogroup pomona 波蒙纳问号血清型钩端螺旋体
- Leptospira interrogans serogroup pyrogene 致热问号血清型钩端螺旋体
- Infection due to Leptospira interrogans 问号钩端螺旋体感染
- Leptospira interrogans serovar Lai 问号赖型钩端螺旋体
- Keywords Leptospira interrogans;DNA chip;detection; 关键词问号钩端螺旋体;基因芯片;检测;
