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- The in vitro and in vivo levels of mIL-12 and the in vivo activity of cytotoxic T lymphocyte(CTL)were examined by ELISA assay and modified LDH release assay,respectively. ELISA法检测细胞上清及小鼠血液中mIL-12水平,LDH释放法检测小鼠体内细胞毒性T淋巴细胞(CTL)杀伤活性。
- Keywords Salvia miltiorrhiza Thymic peptides LDH release assay Psoriasis NK cell; 关键词丹参;胸腺肽;LDH释放法;银屑病;NK细胞;
- LDH release assay LDH微量释放法
- Study on Determination of Cytotoxicity of T-AK Cells with MTT Colorimetry and LDH Release Assays MTT法和LDH法检测T-AK细胞活性的研究
- The tumor specific CTL was generated form activated autologous T cell by DC vaccine loaded with tumor antigen, and the killing activity of CTL to CT26 cells was tested by the lactate dehydrogenase (LDH) release assay in vitro. 负载肿瘤抗原的DC疫苗激活同源T淋巴细胞产生细胞毒性T淋巴细胞(CTL),采用乳酸脱氢酶(LDH)4h释放法检测CTL在体外对CT26细胞的杀伤活性;
- The serum preparation of CSC (2,4,8g/kg) attenuated neuronal damage during hypoxia 3 h / reoxygenation (0,3,6,18 h) by decreasing LDH release. 其含药血清可抑制缺糖 缺氧3h再灌 0h、3h、6h和 1 8h引起的神经细胞内LDH的释放。
- RESULTS: TFP could significantly reduce the tissue calcium , LDH release and protein loss of the myocardium (P0.01) while VP had no such effects. 结果:TFP显著减少损伤心肌的钙含量、LDH及蛋白漏出量,与对照组相比差异显著(P0.;01)。
- An ADCC-51Cr release assay mediated by monoclonal antibodies against herpes simplex virus ( HSV ) was established. 建立了单克隆抗体(McAb)介导细胞毒作用(ADCC)~(51)Cr释放试验的测定力法。
- Exposure of RTE cells to 0.25, 0.5 and 1 mmol/L ONOO - caused a dose-dependent suppression of the mitochondrial respiration . ONOO - also caused a dose-dependent increase in the percentage of LDH release. ONOO-(0 2 5 - 1mmol/L)呈剂量依赖方式抑制RTE细胞线粒体呼吸功能、增高LDH释放率。
- The EC survive rates of haw thorn flavone + oxLDL groups in three doses were higher(P < 0.05), the LDH release rates, MC-EC sticking rates and TBARS were lower( P< 0.05) in a dose-effect manner compared with those of oxLDL group, respectively. 山楂黄酮可以有效地抑制oxLDL对EC的损伤;3个剂量的山楂黄酮+oxLDL组的细胞生存率均显著高于oxLDL组(P<0.;05);LDH释放率、MC-EC粘附率和TBARS值均显著低于oxLDL组(P<0
- EC survive rate of oxLDL group was lower (P<0.01) and LDH release rate was higher(P < 0.05) than those of normal control group. Haw thorn flavone could protect EC from damage caused by oxLDL. oxLDL对EC具有明显的损伤作用;oxLDL组的细胞生存率低于正常对照组(P<0.;05);LDH释放率和MC-EC粘附率均高于正常对照组(P<0
- The peripheral blood nature killer (NK) activity of 58 cases with lymphoma and 52 age and sex matched normal controls was determined by~(125) I-UdR release assay (using K_(562) cells as targets). 应用~(126)I-UdR释放法,检测58例淋巴瘤病人及52例正常人外周血自然杀伤细胞(NK)活性。
- Results After incubation with linoleic acid,pancreatic acinar cells stained with trypan blue were damaged,with LDH release rate at time-and dose-dependent fashion with linoleic acid. 结果腺泡细胞与亚油酸共同孵育后,台盼蓝染色显示存在细胞损伤,LDH释放率与亚油酸呈时间和剂量依赖关系。
- Immune sera from rAAV-2-Ag85A immunized Balb/c mice were tested for anti-Ag85A antibodies by the ELISA method. Cytotoxicity of the T-lymphocyte (CTL) was determined using the ~(51)Cr release assay. rAAV-2-Ag85A免疫Balb/c小鼠,ELISA法检测血清中抗-Ag85A抗体,~(51)Cr释放分析检测细胞毒性T淋巴细胞(CTL)活性。
- Finally, the effect of mPTAl polyclonal antibody on murine T lymphocyte differentiation and cytotoxic function generated from MLC was evaluated with a standard ~(51)Cr release assay. 通过混合淋巴细胞培养(MLC)诱导产生CTL效应细胞,以~(51)Cr释放实验检测mPTA1多克隆抗体在MLC中对T淋巴细胞分化及杀伤功能的影响。
- Methods 3 kinds of T cell lines (OE4, 4Q11, BK1) were used in specific51 Cr release assay, target cell line was TA3. Results CD8+ CTL line OE4 caused highly specific lysis of target cells. 方法采用51Cr释放试验,检测了3种T细胞系(OE4、4Q11、BK1)对瘤细胞TA3的细胞毒作用。
- Serotonin release assay for platelet antibody 血小板抗体5-羟色胺释放测定
- Keywords fibroblast;UV;damage and repair;MTT colorimetric assay;LDH release;green tea;hawk-tea;scutellaria baicalensis georgi;astragalus membranaceus;natural production; 成纤维细胞;紫外线;损伤修复;MTT比色法;LDH;黄芩;黄芪;绿茶;老鹰茶;天然产物;
- We will make an assay of the ore that he brought. 我们将对他带来的矿石做实验鉴定。
- LDH assay showed CTL was induced by the bcr abl immunization. 4bcr- abl基因免疫后的小鼠能有效诱导出特异性细胞毒 T细胞 (cytotoxic T lymph-cyte;CTL) .