It was noted that the binding between NE2 protein and 8H3 can be enhanced by the pre-binding of 8C11. The equilibrium dissociation constants (KD) of these three MAbs and their Fab fragments were determined by BIAcore biosensor.

• 利用Biacore生物传感器测定这3株MAb及其Fab的亲和常数(KD),8C11和13D8的KD值均为20nmol/L左右,其Fab片段的KD值较完整抗体有数十倍的提高,可解释为完整抗体的二价效应;