It was based on the reaction of API with nitrite in acidic medium to form diazo salt and further in alkaline medium to form hydroxydiazene with higher fluorescence intensity.

• API在酸性介质中与亚硝酸根生成重氮盐化合物,进一步在碱性介质中生成相应的羟基二氮烯,使荧光强度明显增强。 基于该反应,提出了一种操作简便、灵敏度高、选择性好的亚硝酸根荧光测定方法。