It shows that the method is suitable for preparation a large amount of safe and effective viral antigen for serodiagnosis of HPAIV.

• 应用1‰终浓度的甲醛可使高滴度的病毒完全灭活，并保持较好的血凝活性，适用于大量制备高致病性禽流感病毒灭活抗原。