Intratype amino acid se-quence variation was assessed. After site-directed mutagenesis of some rare codons near the L1start and final sequence,a typical variation of HPV6L1ORF was cloned into the plasmid pET32a.

• 分析L1区的氨基酸序列变异状况,选择L1序列变异较大的临床分离标本,通过对HPV6L1基因的起始和终止端进行基因定点改造,连于表达载体质粒pET32a,在原核表达体系表达重组HPV6L1蛋白,并进行L1蛋白的抗原性检测。