Interferon-y (IFN-y) and interleukin-4 (IL-4) mRNA expression in PBMC was quantified with TaqMan Real-time PCR using an ABI PRISM 7700 Sequence Detector System (Perkin-Elmer Applied Biosystems).

• mRNA的定量测定是在ABI PRISM 7700序列检测系统上应用TaqMan Real-time PCR技术进行的。