In traditional methods, hepatocytes can not be cultured with high viability and high density in vitro which restrict the evaluation of drug-induced hepatotoxicity in vitro.

• 摘要 传统的细胞培养方法中，肝细胞在体外不能实现高活性高密度的生长，因而无法模拟体内的三维立体生长环境，从而制约了药物肝毒性的体外评价；