In this study, AFB_1 artificial antigen was prepared by 3-mercaptopropionic acid, 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide (EDC) catalytic reaction, and AFB_1 monoclonal antibody immunoaffinity chromatography column was also prepared.

• 本研究主要内容包括:AFB_1人工抗原的制备,AFB_1间接竞争ELISA检测方法的建立,以及AFB_1单克隆抗体免疫亲和柱的研制。