In this paper, to use Cytodex-3 as microcarrier, we established Three-dimensional (3D) culture of rat WB-F344 cells with rotary cell culture system (RCCS) to simulate microgravity environment.

• 本文以大鼠肝干细胞系WB-F344为实验材料,采用RCCS系统模拟微重力效应,并以Cytodex-3为微载体对细胞进行培养(以平面培养的细胞为对照),分析微重力三维培养条件下细胞形态、增殖、细胞内部显微结构及细胞粘附分子表达情况;