In this article, the simple principles and application of affinity chromatography including IAFC, LAFC, DNA/RNA-AFC, IMAC and DAFC as a method for protein research is described.

• 本文介绍了免疫亲和层析、凝集素亲和层析、核酸亲和层析、金属螯合亲和层析以及染料配体亲和层析等几类常规亲和层析的原理及在蛋白质分离纯化以及分析鉴定方面的应用;