In the process of separation and purification of IgM. its actual content in serum was used in calculation instead of calculation by adding purified volume as described by Van Dalen et al and the IgM yield was increased by ten folds.

• 在分离纯化IgM过程中,用IgM实际含有量替代Van Dalen等人原方法中以投入纯化的血清原体积计数法,使IgM得率提高10倍;