In the present study, The part sequences of VP2 segment of PPV GD strain was amplified by PCR and recombinant plasmid contain the VP2 segment was successfully constructed.

• 本研究利用PCR技术扩增出PPV GD株VP2基因序列的一段靶序列进行克隆，构建重组质粒作为标准阳性模板。