In order to prevent cross linking reaction happening between BSA moleculars, the modified bovine serum albumen (M-BSA) was used in the EDC catalytic coupled reaction between AFB_1 and BSA, the coupled reaction was monitored by ELISA assay.

• 在第二步EDC法制备AFB_1肟化物与载体蛋白的偶联物时,采用修饰的牛血清白蛋白(M-BSA),避免了EDC引起的蛋白质自身交联问题。