Immunohistochemical determination of the presence and localization of three basement membrane molecules, collagen IV, laminin, and collagen VII, was conducted for each ECM scaffold.

• 对每一种ECM支架，我们采用免疫组化的方法检测了各自基底膜是否存在，并对IV型胶原、层粘连蛋白以及VII型胶原等分子成分进行了定位处理。