Immunohischemistry stainning, MTT assay and flow cytometry (FACS) were used to detect the expression of proliferating cell nuclear antigen (PCNA), cell proliferation, and cell cycle phase change.

• 采用免疫组化染色法、MTT法和流式细胞仪检测PCNA表达、细胞增殖和细胞周期变化。