Immunofluorescence analysis. Primary antibodies were monoclonal antibodies of p21, cyclin D1, CDK4. FITC-labeled secondary antibodies were used. The fluorescence was measured by Coulter EPICS XL.

• 免疫荧光分析：第一抗体为细胞周期蛋白D1(cyelin D1)、细胞周期蛋白依赖性激酶4(CDK4)、p21蛋白的单克隆抗体，第二抗体采用荧光素标记，应用流式细胞仪检测荧光强度。