ImmunocytochemistryThe BME - induced mMSCs were fixed with 4% paraformalde-hyde, incubated with primary antibodies of NSE and GFAP overnight at 4C , incubated with secondary antibody for 30min.

• brillny acidic protein，GFAP）的表达情况。 2.；3 免疫细胞化学检测诱导后细胞NSE和GFAP的表达将用BME诱导后的细胞爬片用4%25多聚甲醛固定30分钟，·2·0