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- The hybridoma cell strain E3E6 secreting monoclonal antibodies was screened and clo ned by indirect ELISA. 通过用间接ELISA试验筛选、克隆出分泌抗RHDV单克隆抗体的杂交瘤细胞林E_3E_6。
- After immunization of BALB/c mice with purified rabies virus(RV),three hybridoma cell strain against RV named C7,C4 and H3,respectively,were developed after fusion between SP2/0 myeloma cell and the stimulated splenocytes. 将狂犬病病毒ERA株纯化后免疫雌性BALB/c小鼠,取其脾细胞与SP2/0骨髓瘤细胞进行融合,经克隆和间接ELISA筛选,获得3株稳定分泌抗狂犬病病毒ERA株单克隆抗体的杂交瘤细胞株,分别命名为C7、C4和H3。
- Establishment or Hybridoma Cell Strain Secreting Human Hepatocyte Growth Factor 人肝细胞生长因子杂交瘤细胞株的建立
- Identification of hybridoma cell strain of monoclonal antibody of bovine viral diarrhea virus 牛病毒性腹泻病毒单克隆抗体杂交瘤细胞株的鉴定
- Hybridoma cell strain 杂交瘤细胞株
- Establishment of hybridoma cell strains secreting McAbs against Yersinia Pestis F1 antigen 分泌抗鼠疫耶尔森菌F1抗原单克隆抗体杂交瘤株的建立
- In the research, five hybridoma cell lines (1A4,2C7, 3G9 3D5 and 2G3 ) secreting monoclonal antibodies(McAbs) against Aeromonas sobria CR79-1-1 strain were established by the fusion technique. 经单抗亚类试剂盒测定,3株单克隆抗体2G3、1A4和3G9蛋白均为IgG,亚类分别为:IgG2_b,IgG1,IgG1。
- The Balb/c mouse was immunized with antigen of purified avian encephalomyelitis virus strain Van Roekel which was adapt for cell culturing. A hybridoma cell line, which can secret monoclonal antibodies against avian encephalomyelitis(AEV), was obtained. 利用提纯的禽脑脊髓炎病毒 (AEV)VR株CEF适应毒制备抗原 ,免疫BALB/c小鼠 ,应用杂交瘤技术以间接ELISA筛选获得 1株分泌抗AEV(VR)单克隆抗体的杂交瘤细胞。
- Conclusion The hybridoma cell line may be applicable to the diagnosis of HBsAg. 结论筛选出了一株适合临床应用的单抗杂交瘤细胞株。
- Successfully established the islet cell strain steadily expressing FADDdel-GFP. 成功建立了稳定表达FADDdel-GFP的胰岛细胞株;
- Results A hybridoma cell line secreting McAb steadily with high titer was obtained, which was named SU-2000. 结果 得到1株稳定分泌抗体、效价高的杂交瘤细胞株,命名为SU-2000。
- Yuanxing Zhang, Wangfun Fong, Pingpei Yung (1998) Optimization in hybridoma cell culture. 张立,沈红,张元兴*(2000)在动态和静态培养条件下杂交瘤细胞的生长和代谢。
- The hybridoma cell lines could produce the McAbs which was specific to BDV P24 and P40 antigens with the appropriate titers. 杂交瘤细胞系H1和H2可以产生一定效价的单克隆抗体,并对BDV的P24和P40抗原具有特异性。
- The monoclone hybridoma cell line obtained was named SRBC-C, which secreted anti-human B grouping red cell antibody and was used for ABO blood typing. 该株能分泌抗B抗体,与B型红细胞发生凝集,对鉴定ABO血型有一定价值。
- Objective To study the apoptosis of ovarian carcinoma cell strain COC1 induced by arsenic trioxide (As_2O_3). 目的研究三氧化二砷(As2O3)诱导卵巢癌细胞株COC1凋亡的作用机理。
- The effects of glucose, L-glutamine, ammonia and lactic acid on the growth of mouse-mouse hybridoma cell line G_5F_4 were studied. 研究了葡萄糖、谷氨酰胺、氨和乳酸对鼠鼠杂交瘤细胞G_5F_4生长的影响。
- Materials include NG108-15 nerve cell strain,L1 cDNA,anti-L1 antibody:provided by Kanazawa University. 材料为NG108-15神经细胞株、L1cDNA、抗L1抗体:由日本金泽大学神经物性部门提供。
- The results showed that compared with myeloma cell, marked changes were not observed in hybridoma cell in cell energy and cell appearance. 结果表明,与凯骨髓瘤细胞相比,杂交瘤细胞的细胞活力和细胞形态无明显变化;
- PURPOSE:To establish a stable cell strain secreted recombinant tissue-type plasminogen activator(rt-PA). 目的:建立稳定表达人组织型纤溶酶原激活剂(tissue-type plasminogen activatort-PA)的细胞系。
- The hybridoma cell line with the collection number of CGMCCNO.1908 is used for a secrete anti-URG11 monoclonal antibody hybridoma cell line. 所述的保藏号为CGMCCNO.;1908的杂交瘤细胞株用于分泌抗URG11单抗的杂交瘤细胞株。