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- Methods Human gastric cancer cell AGS were cultured in a RPMI 1640 medium with 10% fetal bovine serum(FBS). 方法胃癌细胞株AGS于含10%25胎牛血清的RPMI1640培养液中常规培养,AGS细胞培养24h至对数生长期改用无血清RPMI1640培养基培养24h加入干预药物。
- Recombinant plasmid pshRNA- dnmt1 was transfected into human gastric cancer cell lines AGS to analyze inhibition of gene dnmt1 expression. 转染胃癌细胞株 AGS,用 RT-PCR 法和 Western Blot 法分别检测其对 dnmt1 基因 mRNA 转录水平和蛋白表达水平的影响。
- Objective To investigate the effects of 12-lipoxygenase(12-LOX) and its inhibitor, baicalein, on PKC activation in human gastric cancer cell AGS. 目的研究胃癌细胞中蛋白激酶C(PKC)活化与12-脂氧化酶(12-LOX)的关系。
- Objective To explore the role of proteasome inhibitor 3,4-dichloroisocoumarin(DCI) on apoptosis of human gastric cancer cell line BGC823. 目的:探讨蛋白酶体抑制剂3,4-二氯异香豆素(DCI)对人胃癌细胞系BGC823体外增殖和凋亡的影响。
- Objective To investigate the inhibitory effect of Acanthopanax giraldii Harms Var Hispidus Hoo polysaccharides(AGP)on the human gastric cancer cell. 目的本文对红毛五加多糖对胃癌的抑制作用进行研究。
- Objective To investigate the role of human angiopoietin 1(Ang1) in tumorigenesis and angiogenesis of human gastric cancer cell line SGC7901 in nude mice. 目的 研究人血管生成素 1 (Ang1 )基因转染人胃癌细胞系SGC790 1后 ,对肿瘤细胞生物学特性以及对小鼠体内致瘤力和血管生成的影响 ,探讨其在胃癌发生及血管生成中的作用。
- The purpose of this study was to evaluate the possibility of inducing a productive transfer of the IL2 and IL4 gene into human gastric cancer cell line(GCC). 研究IL2,IL4基因转染人胃癌细胞株分泌IL2,IL4的可行性。
- Objective To investigate the biological effect of CD40 stimulation on human gastric cancer cell lines AGS and BGC-823 ,and analyze the difference of gene expression profiles of AGS after activating CD40 signalling. 目的探讨激发CD40信号对人胃癌细胞株AGS和BGC-823的生物学作用及CD40信号激活前后AGS细胞基因表达谱变化。
- Objective:To investigate the growth inhibition of Oxaliplatin(L-OHP) or Cisplatin(CDDP) in vitro and Livin gene expression induced by the two platinum compounds on human gastric cancer cell line(BGC-823). 目的:研究奥沙利铂(L-OHP)或顺铂(CDDP)对人胃癌细胞株BGC-823生长的抑制作用以及Livin基因表达的变化。
- Objective:Tostudy the effect of human gastric cancer cell multidrug resistance-associated monoclonal antibody on multidrug-resistance(MDR) in Vincristine-resistance human gastric cancer cell line (SGC7901/VCR). 目的:研究胃癌细胞耐药相关性单克隆抗体MGr-1对耐长春新碱胃癌细胞(SGC7901/VCR)多药耐药性(MDR)的影响。
- METHODS: Human breast cancer cell line MCF 7, human gastric cancer cell line BGC 823, human prostate cancer cell line DU 145, and human colon cancer cell line HT 29 were treated with 5 Aza CdR. 方法:用5-Aza-CdR作用人乳腺癌细胞系MCF-7、人胃癌细胞系BGC-823、人的前列腺癌细胞系DU-145和人结肠癌细胞系HT-29。
- MGr-l possesses function of reversing MDR by increasing intracellular adriamycin accumulation and retention, suggesting that MGr-1 Ag may respresent another mechanism responsible for MDR in VCR-resistant human gastric cancer cell line (SGC7901/VCR). MGr-1具有通过增加细胞内药物蓄积及潴留逆转SGC7901/VCR细胞耐药性的功能。 提示MGr-1所对应的细胞膜抗原可能为一种新的有活性功能并与MDR相关的分子。
- Objective:To compare the inhibitory effect of polysaccharrides between Acanthopanax giraldii Harms (AGP) and Lentinan on the proliferatoion of the human gastric cancer cell in vitro. 目的比较红毛五加多糖和香菇多糖对体外人胃癌细胞增殖的抑制作用。
- Objective To study the biological effects of arsenic trioxide(As2O3) on human gastric cancer cells MKN-28 in vitro. 目的探讨三氧化二砷(As2O3)抑制人胃癌细胞株MKN-28生长及诱导其凋亡的生物学效应。
- Objective To observe the effect of telomerase antisense oligodexynucleotides on human gastric cancerous cell line. 目的:观察端粒酶反义寡聚脱氧核苷酸对人胃癌细胞株的作用。
- Conclusion:MACS can be used to isolate human gastric cancer cells with high activity. 结论:免疫磁珠分选法能分离出高活性的人体胃癌细胞。
- Objective To investigate the inhibitory character and the mechanism of Acanthopanax giraldii Harms Var Hispidus Hoo polysaccharides (AGP) on the in vitro human gastric cancer cell line. 目的研究红毛五加多糖对胃癌细胞的抑制作用特点,并探讨该多糖的抑癌机制。
- Abstract: Objective: The aim of this study was to investigate vitro/vivo killing effect of suicide gene CD on the human gastric cancer cell line 7901 by gene transfection method. 摘 要: 目的:通过转基因方法观察自杀基因CD对人胃癌细胞株7901的体内外杀伤作用,并观察组织病理变化。
- MATERIAL AND METHODS: Human gastric cancer cells BGC_823were incubated with various concentrations of sulindac for different periods. 材料与方法:将舒林酸作用于人胃癌BGC-823细胞,并设置不同的作用浓度和作用时间。
- BACKGROUND &AIM: To study the effect of sulindac on growth inhibition of human gastric cancer cells BGC_823in vitro and its antineoplastic mechanisms. 背景与目的:体外研究舒林酸对人胃癌BGC-823细胞的生长抑制作用,探讨其作用机制。