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- Title： Expression of Soluble Human ScFv Against Envelope Protein E2 of Hepatitis C Virus Antigen in E.coli. 关键词：丙型肝炎病毒;包膜蛋白;单链可变区抗体;表达
- We reconstructed the gene of human scFv (VL-VH) and prepared scFv(VL-VH) and scFv (VH-VL) antibody fragments by prokaryotic expression system PET22b(+)/BL21(DE3). 对筛选获得的人源抗G5-24 scFv(VL-VH)基因进行了重构，将它们克隆入表达载体pET22b(+)中，转化大肠杆菌BL21(DE3) ，IPTG诱导表达；
- A hum an phage-displayed scFv library with about 7×10 8 sink size was constructed. A new human scFv was selected, which bound specifically to rabies virus and ha d higher affinity. 结果 :成功地构建了库容量约为 7× 10 8抗狂犬病毒噬菌体scFv库 ,筛选到 1株新的抗狂犬病毒的scFv S12。
- Human ScFv against botulinum neurotoxin serotype A(BoNTa) was modified by fusing human IgG1 Fc to C terminal of ScFv. ScFv-Fc fusion protein was expressed at high level over 30% of total host cell proteins in E. coli. 以获得的抗A型肉毒毒素单链抗体为模板,进行融合改构,将人IgG1的Fc片段连接到ScFv的C端,在大肠杆菌中实现抗体融合蛋白ScFv-Fc的表达,表达量30%25以上,蛋白以包含体形式存在,经过体外变复性的抗体融合蛋白ScFv-Fc,进行Prote in G Sepharose柱亲和层析纯化,纯度达90%25~95%25。
- Screening of Human ScFv Antibody of HCV Helicase 抗HCV解旋酶人源性单链噬菌体抗体的筛选
- Keywords Phage antibody library Human ScFv Digoxin Toxication; 噬菌体抗体库;人单链抗体;地高辛;中毒;
- It's only human nature to want a comfortable life. 想过舒适的生活不过是普通人的本性。
- Ligation of VH and VL during construction of large human ScFv antibody library 大容量人源单链抗体库构建中轻链重链可变区的连接
- Keywords Botulinum neurotoxin serotype A;Phage library;Human ScFv;Expression; 型肉毒毒素;噬菌体文库;人源单链抗体;表达;
- Speeches distinguish human beings from animals. 人类和动物的区别在于人会说话。
- The war took a heavy toll of human life. 这次战争夺去了许多人的生命。
- Perhaps it is our human nature to be nostalgic. 也许是人类天生有一种思古之幽情。
- The priest says that the human spirit never dies. 牧师说，人的灵魂永远不死。
- Human life and turnip remain cheap and plentiful. 人命与萝卜依然是多而且便宜。
- He took a mournful view on human affairs. 他对人世持悲观的态度。
- She was destitute of human feeling. 她一点也没有人的感情。
- Human nature revolts at such a crime. 人性厌恶这样的罪行。
- They listened to his discourse on human relations. 他们听他作关于人际关系的演讲。
- Human tissue is made up of cells. 人体的组织是由细胞构成的。
- The accident was caused by human error. 这宗事故是人为过失造成的。
