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- Methods: (1)To detect toxic effect of PAP to Hela cell by MTT assay. 方法:(1)用MTT法检测PAP对Hela细胞的毒性作用;
- KGF and KGFR are expressed in Hela cells. Hela细胞中有KGF和KGFR的表达;
- Low dose cisplatin can enhance the sensitivity of radiotherapy through change cell cycle of Hela cell lines. 小剂量顺铂可通过改变培养的人Hela细胞周期 ,诱导细胞G2 /M期延迟从而提高放疗敏感性 ,可能与其内在的 p5 3基因表达改变有关。
- Aim To evaluate the manner and molecular mechanism of HeLa cell death induced by Coxsackie virus B3(CVB3). 目的评价柯萨奇病毒B3CVB3致HeLa细胞死亡的方式及分子机制。
- Role of Mitochondria in HeLa Cell Apoptosis Induced by Hematoporphyrin monomethyl ether- PDT[J]. 引用该论文 丁新民;刘凡光;顾瑛;曾晶;戴维德;李晓松.
- Stably transfected and control Hela cell lines were injected subcutaneously into female Balb/c/nu mice. 稳定转染Ang-2的细胞注射Balb/c裸鼠皮下。
- In HeLa cell cytotoxicity test, after incubate 72 hr all of Paclitaxel-loaded particles have cell inhibition effect. 最后作了HeLa细胞毒性测试，包了药的无论是微胞或水胶粒子，在培养72hr都可看到明显抑制癌细胞效果。
- Ang-2 cDNA were subcloned into pcDNA3 vector and transfected into Hela cell lines by the lipofection method. 将Ang-2的cDNA克隆到真核表达载体pcDNA3上，应用脂质体转染技术将该真核表达载体稳定转染人Hela细胞。
- Hela cells were also injected to normal BALB/c and Kunming mice. 同时用Hela细胞作为阳性肿瘤细胞对照，并接种于免疫功能正常的BALB/c小鼠和昆明种小鼠。
- HPV16 E7 was expressed efficiently in HEK-293 and HeLa cells. 荧光显微镜下,转染后HEK-293细胞和HeLa细胞表达绿色荧光蛋白。
- To observe direct inactivate effect of PAP to CBV3 by microdose cytopathogenic effect inhibition assay in Hela cell cultures. 在Hela细胞上采用微量细胞病变抑制法观察PAP直接灭活CBV3的作用。
- Results All the test results showed that M-PPC induced no cytotoxicity to Hela cell, and M-PPC had good biocompatibility in vivo and in vitro. 结果制备出M-PPC；M-P代无细胞毒性，植入白兔背部肌肉后，机体无任何炎症反应。
- Objective: To study the fate of LAK cell and rescuing effect of Astragalus polysaccharide (APS) during and following assault on Hela cells. 目的：进一步探讨LAK细胞攻击Hela细胞后的归宿及黄芪多糖（APS）的影响。
- We measured HeLa cell intracellular Ca2+ transfected with constructsbearing overexpressing BRI3 and CAML respectively. The results showed that BRI3overexpression could increase the cytoplasmic Ca2+ as well as CAML gene did. 我们在HeLa细胞中过表达BRI3基因和CAML基因，并测定了PMA存在的情况下，胞内游2+离Ca的浓度，结果发现和CAML一样，BRI3基因的过表达也可以提高HeLa2+2+细胞内游离Ca浓度。
- Flow cytometry (FCM) was used to examine the effects of 4-HPR with or without DDP on cell cycle and apoptosis of HeLa cells. 采用流式细胞仪测定4-HPR、DDP、4-HPR+DDP对HeLa肿瘤细胞系细胞增殖周期和细胞凋亡的变化。
- In the second section, in vitro experiments were conducted to investigate the effects of Dex, Tween-80, Ca~2+, EDTA and Aminophylline on HeLa cell proliferation, apoptosis and its HSP70 expression. 本试验以地塞米松(dexamethasone,Dex)、吐温-80(Tween-80)、氨茶碱(Aminophylline)、乙二胺四乙酸(EDTA)、Ca~(2+)、双氧水(H_2O_2)为应激源,旨在探讨不同应激处理下HeLa细胞凋亡、增殖及HSP70表达的特点和规律,以及HSP70可能的表达通路。
- Methods:The proteins of HeLa cell were extracted by three different lysis buffers to do 2-DE. The gel images then were acquired by Amersham ImageMaster VDS-CL to do the comparative analysis with PDQuest(7.4.0)software. 方法:用三种不同配方的裂解液提取HeLa细胞中的蛋白质;进行双向凝胶电泳;用Amersham ImageMaster VDS-CL型凝胶成像系统获取凝胶图像;以PDQuest(7.;4
- RT-PCR showed that E1A gene had a stable transfection in Hela cells and obviously down-regulated Her-2 expression. RT-PCR结果显示:E1A基因在Hela细胞中能稳定表达,E1A基因的转染明显降低了Her-2基因在Hela细胞中的表达水平;
- At 12 h, the H_2O_2 level in HeLa cells and AsPC-1 cells were similar to that of the control group. 结论:As2O3诱导HeLa细胞和AsPC-1细胞凋亡与细胞内的H2O2水平改变有关,而与HPV的感染无明显相关性。
- The s-lap/GFP fusion protein can express in Hela cells and locate in entire cells, but express highly in the nucleus. s- lap/ GFP融合蛋白可在人 Hela细胞中表达 ,并主要定位于细胞核。
