HSF and HSC-T6 in logarithmic growth phase were incubated in culture plates in which the cultured cell density were adjusted to 1 x lOVml. OD values of the cells were tested by the method of MTT after 24hr incubation.

• 取对数生长期培养细胞HSF，HSC一T6，调整细胞密度为lxl05个/m1，接种于%25孔板24小时后，分别加入不同浓度pHGF，孵育24小时，进行MTT试验，检测55Onm0D值。