HRP or labelled HRP catalyzes the oxidation reaction of MAP with H2O2, the product of which yields a sensitive voltammetric peak at potential of - 0.46V ( vs. SCE) in Britton -Robinson (B - R) buffer solution.

• 本方法以线性扫描二阶导数伏安法检测HRP催化H_2O_2氧化MAP的产物；用于游离HRP和各种HRP标记物的测定；灵敏度均高于经典的ELISA显色光度法.