Genomic DNA was obtained from Pleurotus sapidus using CTAB extraction. The nuclear ribosomal ITS was amplified with primers ITS1 and ITS4, and PCR products were purified with a Wizard PCR purification kit.

• 用CTAB法提取美味侧耳基因组DNA,利用引物ITS1和ITS4扩增其核糖体DNA的ITS片段,凝胶回收试剂盒纯化目的片段。