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- Gelatinase zymography 明胶酶谱分析
- In this study, there was no change in MMP2 (gelatinase A) production. 在这项研究中，产物MMP2并没有多大改变。
- Conclusion Gelatinase A may play an important role in the progression of primary hepatocellular carcinoma. 结论明胶酶A在原发性肝癌的进展中可能起重要作用。
- Objective To investigate the effect of HCMV infection on the activity of gelatinase in cultured human embryo lung fibroblast( HEL). 目的探讨人巨细胞病毒(CMV)染对体外培养肺成纤维细胞(hel)明胶酶活性的影响。
- The corn gelatin starch prepared in pilot plant scale was used to substitute gelatinas a stabilizer in ice cream and fermented milk drink and as a gelatinizer in fruit jelly. 采用玉米凝胶淀粉的中试产品，作果冻胶凝剂，冰淇淋、发酵乳酸饮料等的增稠稳定剂。
- Objective To investigate the effect of HCMV infection on the activity of gelatinase in cultured human embryo lung fibroblast( HEL ). 目的探讨人巨细胞病毒(CMV)染对体外培养肺成纤维细胞(hel)明胶酶活性的影响。
- Firstly, both MPO and DPO activities in all three groups were identified andmeasured by zymography following native PAGE at 5,7,11 and 15 days,respectively. 首先，经非变性PAGE分离后，采用酶底物显色方法，鉴定并测定了血餐后d_5、d_7、d_(11)和d_(15)三组雌大劣按蚊血淋巴MPO和o-DPO酶活性。
- The studies indicated that the main compositions of Gelatina Nigra are protein,polypeptides,animo acids,mental elements,dermatan sulfate,biotic acids and so on. 近年有关阿胶成分及质量评价研究文献表明,阿胶主要成分为蛋白质水解产生的多肽、多种氨基酸、金属元素及硫酸皮肤素、生物酸等。
- The results showed that blood-brain barrier dysfunction occured in ducks infected with RA and the gelatinase was important to the dysfunction. 表明，鸭疫里默氏杆菌感染引起鸭血-脑屏障机能障碍，在此过程中明胶酶起着重要作用。
- The effect of CsA on the transcription and translation of MMP-9 and MMP-2 was determined by Reverse Transcription-Polymerase Chain Reaction(RT-PCR)and Gelatin Zymography. RT-PCR与明胶酶谱分析CsA对滋养细胞MMP-9与MMP-2 mRNA及蛋白水平表达的影响;
- Total RNA was isolated and gelatinase A (MMP2) gene expression level were rneasured by reverse transcription polymerase chain reaction (RT-PCR). 提取组织总RNA；用逆转录共扩增定量PCR方法测定明胶酶A（MMP2）基因表达水平。
- Their abilities to produce extra-cellular protease, gelatinase, phospholipase, amylase as well as ability to break down goat blood cells were investigated. 结果表明，虽然来自下沙病鲍苗的菌株比来自官湖的健康鲍苗的溶血能力差；
- A possible role for MMP-2(gelatinase A) and MMP-9(gelatinase B) in acute leukemia has been hypothesized, because of the role of MMP-2 and MMP-9 in tumor. 研究发现，许多细胞因子对MMP-2和MMP-9有调节作用，反之亦然。MMP、其天然抑制剂TIMP和微环境中的细胞因子三者之间相互调节，构成一个交叉网络而发挥其功能。
- RT-PCR was used to estimate the relative mRNA amounts of estrogen receptor(ER),MTA3 and snail in the cells. Western blotting and gelatin zymography assay were used to estimate the relative protein amounts of MMP-2,MMP-9 and E-cadherin in the cells. 采用RT-PCR技术检测雌激素受体(ER)以及与侵袭有关的MTA3、Snail基因mRNA的表达,采用蛋白印迹法和明胶酶谱法检测E-钙黏附蛋白(E-cadherin)和基质金属蛋白酶2(MMP-2)和9(MMP-9)蛋白的表达。
- In addition, the interaction between matrix m etalloproteinases (MMPs) and ET-1 was investigated by cel l transfection with anti-ET-1 converting enzyme (anti-ECE) mRNA expression plasmid followed by gelatin zymography analysis. 将细胞转染反义内皮素转换酶(anti-ECE)mRNA表达载体并运用酶谱分析法,研究ET-1与基质金属蛋白酶(MMPs)的相互作用。
- Methods: The enzymatic activities of MMP-2 and MMP-9 were detected by gelatin zymography in 50 cases of human brain gliomas and 4 cases of normal human brian tissues. Immunohistochemical study was used for MMP2 and MMP-9 expression in all cases. 方法:采用明胶酶谱与免疫组化方法对50例人脑胶质瘤和4例正常脑组织中的MMP-2及MMP-9进行检测。
- Film in situ zymography demonstrated a high level of protease activity in follicle cells from starved females, whereas only a low level of activity was detected in females with developing ovaries. 利用原位酵素图谱证明，在饥饿雌虫的滤泡细胞内有高程度的蛋白酶活性，而在卵巢正在发育的雌虫中，只侦测到低程度的活性。
- CONCLUSION: The tissue environment has definitive effects on the tumor phenotypes, and the expression of gelatinase is closely related to the growth pattern and invasion of tumor cells. 目的: 探讨不同组织微环境中明胶酶在异种移植瘤中的表达差异及其对移植瘤生物学行为的影响.
- Keywords breast neoplasms;gelatinase A;zymography analysis;neoplasm invasiveness;neoplasm metastasis; 乳腺肿瘤;明胶酶A;酶谱分析;肿瘤侵润;肿瘤转移;
- Keywords Henoch-Schonlein purpura nephritis;gelatinase A;gelatinase B;zymography; 肾炎;紫癜性;明胶酶A;明胶酶B;明胶酶谱法;
