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- Objective To investigate the effect of bcr abl fusion gene on CML cell apoptosis. 目的研究bcr?abl融合基因在慢性粒细胞白血病细胞（CML）凋亡调控中的作用。
- A rapid reverse transcriptase transcribed PCR(RT PCR) was established to detect different types (b3a2, b2a2, B1a2) of bcr abl fusion mRNAs in leukemias. 建立白血病中费城（Ph）染色体上独特的bcr－abl融合基因的逆转录PCR（RT－PCR）快速检测方法。
- Firstly, three hammer head ribozymes specific to the fusion point of bcr abl were designed and cloned into a modified in vitro transcription vector pDES. 为此，我们针对bcr?abl 融合位点设计、合成了3 个相邻的锤头状核酶。
- The PTK activity of BCR ABL protein was also mildly decreased in CML monouclear cells at 60 h. As2 O3作用 6 0h ,可使CML患者骨髓单个核细胞BCR ABL蛋白PTK活性轻度下降。
- Objective To explore the effects of As 2O 3 on BCR ABL protein level and signal transduction in chronic myeloid leukemia(CML) cells. 目的 阐述As2 O3对慢性粒细胞白血病 (CML)原代细胞BCR ABL及信号转导的影响。
- Purified fusion protein was obtained. 得到纯化的融合蛋白。
- LDH assay showed CTL was induced by the bcr abl immunization. 4bcr- abl基因免疫后的小鼠能有效诱导出特异性细胞毒 T细胞 (cytotoxic T lymph-cyte;CTL) .
- The results showed that better reaction conditions were gained by exploration of hybridizotion temperature and elution conditions, bcr abl fusion gene in leukemia cells was detected by prepared miccroarray. 结果表明 :通过对不同杂交温度、洗涤条件等的探索获得了较好的反应条件并用制备的芯片检测出了细胞株中的bcr abl融合基因。
- Secondary, a 376bp fragment containing the bcr abl fusion site was amplified by PCR. This fragment was cloned into pBluescript KS to be used as the template vector for in vitro cleavage experiment. 此外， 通过PCR 方法， 扩增了bcr?abl 融合位点附近约376bp 的序列，成功地构建了bcr?abl 融合基因体外转录载体。
- Method:The fragement of Bcr abl fusion gene was amplified from K562 cells of CML by RT PCR and was cloned into the downstream of GST gene in pGEX 6P 1 vector according to the current open reading frame. 方法 :以慢性粒细胞白血病 (CML)K5 6 2细胞株总RNA作为模板 ,采用RT PCR方法扩增包含Bcr abl(b3a2 )融合位点周围的基因片段。
- The GRA7 gene may be expressed as a GST fusion protein in E. coli. GRA7基因在大肠埃希菌中以GST融合蛋白的形式得到表达。
- The fusion protein was purified by GSTrap FF affinity chromatography. GSTrap FF亲和层析柱纯化融合蛋白;
- The fusion protein was purified through Nickel-affinity chromatography column. 用镍柱纯化融合蛋白。
- Methods The recombinant fusion protein MBP hEra was expressed in the E. 方法在大肠杆菌中,表达重组MBP?hEra融合蛋白。
- Apoptosis of K562 cells was significantly increased associated with inhibition of bcr abl expression. bcr?abl融合基因表达受抑后，K562细胞凋亡显著增加。
- Mimics of both types of BCR ABL cDNA were achieved and the validity was verified with restriction endonuclease. 经酶切分析证明 ,两型BCR ABLmRNA均可通过此方法得到相应的cDNA参照物。
- The obtained recombinant vector, named pGEM mimic, can be used as a common competitor for either b3a2 or b2a2 type of bcr abl cDNA. 较b2a2型bcr－ablcDNA相应的201bp扩增片段长39bp，使之适于作为b3a2和b2a2型两类bcr－ablmRNA定量PCR的通用内标物。
- BVP22 can mediate intercellular trafficking of fusion protein in vitro and in vivo. 在体内外BVP22均能够介导与其融合的蛋白在细胞间发生穿梭。
- Immunoprecipitation and Immunoblot have found that WT, Y279F, Y304F and Y546F Syp can bind directly to the Bcr Abl in vivo. 免疫沉淀与免疫印迹结果发现WT、Y279F、Y304F和Y546F等4 种Syp 在胞内均能直接与Bcr?Abl 结合。
- Among 13 cases with BCR ABL assay,all 8 cases with Ph positive were BCR ABL positive and in 5 cases with Ph negative 2 (40%) were BCR ABL positive. 8 例 Ph（＋ ）患者 B C R? A B L基因亦阳性，5 例 Ph（－ ）患者有2 例（40%25 ）阳性；
