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- Two EST(One come from hemin induced HEL cells while the other come from PMA induced HEL cells) were chosen as the probes for cDNA library screening and several full length cDNA clones were isolated from human bone marrow cDNA library. 选择两个新EST筛选人骨髓细胞cDNA文库，获得两个新的锌指蛋白编码基因的全长cDNA，分别命名为HZF1(其EST来源于被hemin诱导的HEL细胞)和HZF2(其EST来源于被PMA诱导的HEL细胞)。
- The Methods of Constructing the Full Length cDNA Library 全长cDNA文库的构建方法
- full length cDNA library 全长cDNA文库
- Objective: To get human CD40 gene full length cDNA sequence and to express functional CD40 molecule in COS 7 cells. 目的：克隆编码人CD40 分子基因的全长cDNA，在COS－7 细胞中获得高表达。
- The full length cDNA of ANGPTL4 consists of 1943 nucleotides in which there is a 1218 bp ORF encoding a 406-amino acid polypeptide (predicted molecular mass is 45.2 kDa). 并用RACE-PCR获得其全长。该基因cDNA全长为1943 bp，开放阅读框含1218 bp，编码406个氨基酸，预测分子量为45.;2 kDa。
- The full length cDNA of the genome segment (S9) of Rice gall dwarf virus Xinyi isolate (RGDV-C) was cloned and the complete nucleotide sequence was determined. 应用RT-PCR技术克隆了水稻瘤矮病毒(RGDV)中国广东信宜分离物(RGDV-C)的基因组S9片段，测定了全序列并进行了生物信息学分析。
- The full length cDNA of Rtn-T was 3491 bp and encodes a 108 kDa protein of 986 amino acids. This Rtn-T has Genbank accession number AF333336. Blast search in the human genome datebase showed that Rtn-T consisted of 9 exons. 序列测定显示Rtn-T cDNA全长为3419bp，编码蛋白为108KDa，含986个氨基酸，该基因已被Genbank收录，收录号为AF333336。
- In this study, the full length cDNA and DNA of pollen development related BcMF2 and BcMF4 gene was cloned and characterized from Chinese cabbage pak-choi (Brassica campestris L. ssp. chinensis Makino). 本研究以BcMF2和BcMF4基因为研究对象,通过PCR扩增方法对白菜BcMF2和BcMF4基因的基因组全长和cDNA全长进行了克隆、鉴定以及蛋白质结构和功能的预测;
- Four full length cDNAs of hatching enzyme genes were cloned from gynogenetic gibel carp ( Carassius auratus gibelio) by suppression subtractive hybridization, SMART cDNA synthesis and RACE PCR. 用抑制性差减杂交结合SMARTcDNA合成和RACE PCR技术 ,克隆得到雌核发育银鲫 (Carassiusauratusgibelio) 4种孵化酶基因的全长cDNA。
- Full length HCLP-1 cDNA was isolated from human fetal brain cDNA library with library screening using the HCF-homological EST probe. HCLP-1 is comprised of 406 我们在酵母双杂交实验中检测HCLP-1是否与HCF的相互作用蛋白VP16和LZIP等转录调控因子相互作用。 随后我们又应用
- Cloning of full length cDNA sequence of the mouse ameloblastin 小鼠成釉蛋白基因cDNA全序列的克隆
- The boy lay at full length on the grass. 男孩伸直身体躺在草地上。
- Construction of Plant Expression Vector for The Full Length cDNA of AIMV Replicase Gene 苜蓿花叶病毒复制酶基因全长cDNA植物表达载体的构建
- Sequence analysis of full length cDNA of Schistosoma japonicum egg miracidia genes harboring signal sequence 日本血吸虫虫卵毛蚴中带信号序列基因的全长cDNA序列分析
- 4 HPH is also a MADS-box gene, the full length cDNA is 888bp. It encodes a putative protein with 210 amino acids. HPI1亦为MADS-box基因，其cDNA全长888bp，编码210个氨基酸。
- Keywords Porcine reproductive and respiratory syndrome virus;Full length cDNA clone;High-pathogencity;Regression text; 猪繁殖与呼吸综合征病毒;感染性克隆;高致病性;动物回归试验;
- Methods Full length HMGB1 cDNA cloned by PCR from mammary library was inserted into pET28a(+) vectors to construct pET28a(+)-HMGB1 plasmid. After confirmation, the positive pET28a(+)-HMGB1 recombinant plasmid was transformed into E. 方法应用PCR从乳腺文库中扩增得到HMGB1基因片段,并将其克隆到pET28a(+)载体上,对获得的pET28a(+)-HMGB1重组质粒扩增后进行PCR、酶切和测序鉴定。
- An Improved Method for Solid-phase cDNA Library Construction. 一种改进的cDNA文库固相构建法。
- Stretched out or lying at full length along the ground; prone. 平伸的整个长度伸展或平放在地面上的; 与地面齐平的
- Construction of cDNA library from Sophora alopecuroides L. 苦豆子cDNA文库的构建。
