Fragment of gyrA and parC gene including the quinolone-resistant determined region (QRDR) were amplified by PCR and cloned ,sequenced.

• 实验选择了动物源、人源的对氟喹诺酮类药物呈不同耐药程度的菌株和敏感菌株，分别提取各菌株的染色体DNA，并对gyrA基因和parC基因进行了聚合酶链式反应（PCR）和克隆测序。