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- Objective To detect Plasmodium falciparum with the Fluorescent Quantitative PCR(FQ PCR) and value this method. 目的评价荧光定量聚合酶链式反应(FQ-PCR)检测恶性疟原虫的效果。
- Real-time fluorescent quantitative PCR basing ordinary PCR is a new technique which possess characteristic of sensitiveness,quickness and speciality,can accurately fix the quality. 实时荧光定量PCR是在普通PCR基础上发展起来的新技术,在保持了PCR技术灵敏、快速、特异特点的基础上增加了定量的特点。
- Real-time fluorescent quantitative PCR(FQ-PCR) is a kind of techniques that can quantitate the nucleic acid based on different fluor escence. 实时荧光定量PCR技术是一种利用不同的荧光检测来给核酸定量的技术。
- real-time fluorescent quantitative PCR 实时荧光定量PCR
- fluorescent quantitative PCR (FQ-PCR) 荧光定量PCR
- fluorescent quantitative PCR method 实时荧光定量PCR方法
- Fluorescent quantitative PCR(FQ-PCR) 荧光定量PCR
- real time fluorescent quantitative PCR 实时荧光定量PCR
- real-time fluorescent quantitative PCR (FQ-PCR) 实时荧光定量PCR
- Realtime fluorescent quantitative PCR RTQ-PCR
- Fluorescence quantitative PCR combined with RT-PCR technique can detect the DTC in blood of the patients with HCC. RT-PCR联合荧光标记探针杂交法能够检测到肝癌患者血液中播散的肿瘤细胞,并且以肺动脉血中阳性率最高。
- Methods HBV markers and HBV-DNA level in the serum were detected by ELISA and fluorescent quantitated PCR. 方法:通过ELISA法及荧光定量PCR法检测血清标本中HBV标志物和HBV-DNA含量水平。
- Objective To evaluate the clinical value of fluorescence quantitative PCR(FQ-PCR) technique in diagnosing tuberculosis. 目的探讨荧光定量聚合酶链反应(FQ-PCR)技术诊断结核病临床应用价值。
- Objective:To construct recombinant plasmid pMD18-nm23-H1/p as the standards for nm23-H1 gene detection by real-time fluorescence quantitative PCR. 目的:制备用于nm23-H1基因mRNA实时荧光定量PCR检测的质粒标准品。
- Methods Pre-S1 antigen of hepatitis Bvirus with ELISA method, it is ultraviolet that ALT use LD method, HBV-DNA was determined by fluorescence quantitative PCR(FQ-PCR) assay. 方法乙肝病毒前S1抗原用ELISA方法检测,丙氨酸氨基转移酶用紫外-乳酸脱氢酸法检测,HBV-DNA测定运用荧光实时定量检测方法。
- Method:Serum samples of 106 suspected HBV patients were detected by MEIA and positive samples were then detected for HBV DNA by Fluorescence quantitative PCR. 目的:应用微粒子荧光酶免疫分析法(MEIA)定量检测乙肝病毒e抗原的浓度,以反映乙肝病毒感染的程度。
- Fluorescent quantitative PCR with complex probe technique 复合探针荧光定量PCR方法的建立
- Methods A model of animal experiment,a kit for sexdeter mining region Y (SRY) and a set of standard sample were developed for drawing standard curve by real time fluorescence quantitative PCR. 方法结合动物实验模型,设计性别决定因子Y(SRY)实时荧光定量聚合酶链反应(PCR)检测血细胞嵌合率的方法,建立质控标准品,使用实时荧光定量PCR技术,获得标准曲线。
- Methods: Fluorescence quantitative PCR (FQ - PCR) was used to detect quantitatively UU DNA and CT DNA in randomized in 513 cases NGU. 方法 用荧光定量多聚酶链式反应(FO-PCR)技术对513例非淋患者进行了UU DNA和CT DNA定量检测。
