Flow cytometric enumeration of CD34 +hematopoietic stem and progenitor cells by double fluorescent labeling, and cell cycle detection by PI labeling at the indicated detection time.

• 在不同时相点,双荧光标记测定CD34+细胞在骨髓有核细胞中的比例,骨髓细胞固定后PI染色测定细胞周期。