Firstly, when RNA polymerase is moving on a DNA template, transcription can result in negative supercoiling behind the polymerase, thus facilitating Z-DNA conformation at permissive regions.

• 形成Z-DNA的原因有很多：首先，转录过程中，移动的RNA聚合酶在模板DNA的5'端产生负超螺旋扭曲力，导致Z-DNA的形成；