Fipronil(F) and one of its analogy(FH)were adopted as haptens0 The former was coupled to carrier protein by both EDC and GD, and the later was coupled to carrier protein by active ester.

• 本文采用氟虫腈（F）及其一种类似物（FH）作为半抗原，分别通过碳化二亚胺法（EDC）和戊二醛法把氟虫腈偶联到载体蛋白上，通过活性酯法把FH偶联到载体蛋白上，作为人工抗原。