FDR3 was inserted into the yeast expression vector pDBLeu and then was transferred to the yeast strain M3(ctrl mutant) mediated by PEG/LiAc and by electroporation .

• 把FDR3基因片段克隆到酵母表达载体pDBLeu上，然后通过电击和乙酸锂转化两种方法将重组质粒导入酿酒酵母Ctrl突变体（M3 mutant）中，采用酵母异源互补法进一步鉴定FDR3基因的功能。