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- Fig.1.Construction of the shuttle expression vector pRL_hEGF. 标题: 图1.;穿梭表达载体pRL_hEGF的构建。
- Objective:To construct an eukaryotic expressing vector pIRES1neo/hFL and express hFL in COS 7 cell. 目的 :构建人FL真核表达载体 pIRES1neo/hFL ,观察其在COS 7细胞中的表达。
- The pPIC9K/Ang-1 yeast expression vector was constructed successfully. 成功构建pPIC9K/Ang-1毕氏酵母表达载体。
- Aim To construct a CHO cell expression vector carrying coamplification gene. 目的构建携带共扩增基因的CHO细胞表达载体。
- The cDNA of BcpLH gene was cloned into a His-fusion expression vector pET-28a (+) and was induced to express in E. colt strain BL21(DE3). 在含有His标记序列的原核表达载体pET28-a(+)上插入Bc-pLH基因的cDNA,在大肠杆菌BL21(DE3)中诱导表达出了特异性蛋白,并免疫大白兔制备出高效价的抗血清;
- Objective To construct endothelial cell-specific molecular-1(ESM-1) eukaryotic express vector,transfect it into human umbilical vein endothelial cells( HUVECs,ECV304) and express it in vitro . 目的 构建人内皮细胞特异性分子 1(ESM 1)真核表达载体 ,转染人脐静脉内皮细胞系ECV30 4 ,并在ECV30 4中获得表达。
- Objective: To construct a procaryotic expression vector of vascularendothelial growth factor 165 (VEGF165) and to express VEGF165 inE. coli. 目的:构建人血管内皮生长因子 165(Vascular endothelial growthfactor 165,VEGF165)原核表达载体并在大肠杆菌中诱导表达。
- Objective:To construct and express a recombinant eukaryotic expression vector bearing fusion gene of human IL-2 cDNA gene and Fc fragment. 目的 :构建含人IL 2cDNA基因和免疫球蛋白Fc片段融合基因的真核表达载体pcDNA 3.;1IL 2 /Fc;并在真核细胞中表达;以期用于乙型肝炎病毒 (HBV)DNA疫苗的研究。
- AIM: To clone human IL-24 (hIL-24) gene and construct its eukaryotic expression vector, then transfect it into Ca Ski cells to express hIL-24 protein. 目的:克隆人IL-24基因并构建真核表达载体;转染Ca Ski细胞进行真核表达.
- Aftertransected with the expression vector , CHO cells can express the fusion protein stably , which was con-firmed by Western blot and ELISA. Western blot及ELISA法检测到细胞培养上清中有融合蛋白的表达。
- The L ScFv genes were inserted into the eukaryotic fusion protein expression vector pEGFP N3 and transfected into COS 7 cells to express respectively. 将所构建的单链抗体基因克隆入绿色荧光蛋白 (GFP)融合表达载体pEGFP N3,并转染COS 7细胞进行表达。
- Abstract: Objective To construct the eukaryotic expression vector which express the protein of VZV glycoprotein E in COS7 cells. [摘 要] 目的 构建水痘-带状疱疹病毒(VZV)糖蛋白E的真核表达载体并使其在COS7细胞中表达。
- Construction of the plant express vector of Pto gene pCAM-P, a plant express vector with Pto gene which was a protein kinase gene conferring disease resistance in tomato was constructed. Pto基因植物表达载体的构建 以Pto基因为目的基因,在启动密码子处设计上游引物,含一个BamH I酶切位点和一个“AACC”真核表达增强序列;
- LCB1 gene was cloned into inducible expression vector pYES2 and transformed into Saccharomyces cerevisiae FY2 The plasmid was induced to express with galactose. 酿酒酵母 (Saccharomycescerevisiae)LCB1 (Longchainbase)基因被克隆到酵母诱导表达载体pYES2中 ,并转入到FY2中 ,用半乳糖诱导表达。
- And then the CED-9 gene was inserted into plant expression vector pBI121 under the control of CaMV 35S promoter. 在此基础上再构建重组表达载体pBI-ced9,将CED-9基因置于CaMV35S启动子控制之下。
- The baculovirus expression vector pAC-HBs-Fc for complete IgG antibody against HBsAg was successfully constructed. 已成功构建表达载体pAC-HBs-Fc,为表达抗人HBsAg的IgG全抗体奠定了基础。
- The cDNA was subcloned into prokaryotic expression vector pET3d and overexpressed in E. coli BL21(DE3). 将该cDNA插入原核表达载体pET3d并在大肠杆菌BL21(DE3)中过量表达。
- Objective To construct the antisense expression vector of human Na + H + exchanger 1 (NHE 1). 目的 构建人Na+ H+ 交换蛋白 1(Na+ H+ exchanger 1,NHE 1)基因反义真核表达载体。
- Eukaryotic expression vector pCDNA3-AADC can be efficiently expressed in primary muscle cells. 含人类神经元AADC基因的真核表达重组体可在原代培养的骨骼肌细胞中有效的表达。
- No words can express the grandeur of that parade. 阅兵式那宏伟的场面是无法用语言表达的。