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- Pichia pastoris expressing system,which is a eukaryotic expressing system developed in recent years,has a promising prospect. 巴氏毕赤酵母表达体系是近年发展起来的真核表达体系,具有良好的应用前景。
- CB could be expressed in high yield by vaccinia virus eukaryotic expression system. 痘苗病毒真核表达系统可以高效表达目的蛋白。
- In the present study, molt-inhibiting hormone-1 from Eriocheir japonica sinensis (Ers-MIH1) was studied both in prokaryotic and eukaryotic expression system. 第二章:中华绒螯蟹蜕皮抑制激素1(Ers-MIH1)基因的原核表达载体的构建
- Eukaryotic expressing system 真核表达系统
- Construction of eukaryotic expression system of staphylococcal enterotoxin A gene and identification of the target recombinant protein 葡萄球菌肠毒素A基因真核表达系统的构建及其目的重组蛋白的鉴定
- Signal peptides' structures and functions, signal sequence traps and the proteins expressions in prokaryote and eukaryote expression systems were illustrated in the article. 就信号肽的结构和功能,信号肽的捕获方法及其在原核表达系统和真核表达系统中表达外源蛋白质的应用做一些介绍。
- Objective:To construct an eukaryotic expressing vector pIRES1neo/hFL and express hFL in COS 7 cell. 目的 :构建人FL真核表达载体 pIRES1neo/hFL ,观察其在COS 7细胞中的表达。
- Construction of Eukaryotic Expression System of Helicobacter Pylori napA Gene and its Expression in Mice 幽门螺杆菌napA基因真核表达系统的构建及其在小鼠体内的表达
- Construction of An Eukaryotic Expression System pCI -PIA Carrying the Porin IA Gene from the Neisseria Gonorrhoeae and Sequence Determination 淋病奈瑟菌孔蛋白PIA真核表达系统的构建及序列分析
- The eukaryotic regulating expression system Tet-Off has Potentiality in gene therapy on the neurodegenerative disorders of CNS, such as AD, when using hNT-3 or other genes. 在应用hNT-3进行AD等中枢神经退行性疾病的基因治疗中,Tet-off调控表达系统具有潜在的应用前景。
- Objective To construct mouse granulocyte-macrophage colony stimulating factor (mGM-CSF) gene eukaryotic expressing plasmid pcDNA3-GM-CSF, to transfect the recombinant into erythroleukemia cell line FBL-3, and identify their biological activity. 目的 构建小鼠转粒细胞 巨噬细胞集落刺激因子 (mGM CSF)真核表达质粒 pcDNA3 GM CSF ,转染红白血病细胞系FBL 3,并鉴定其活性。
- Objective To construct the antisense nucleic acid eukaryotic expressing vector of the cDNA sequence encoding the transmembrane domain of receptor tyrosine kinase RON (RON-RTK) gene (RONm). 目的构建RON基因跨膜区段(RONm)的反义核酸真核表达载体。
- The recombinant eukaryotic expression plasmid,pcDNA3.1A DCN was successfully constructed and 2 cell clones positively expressing DCN were selected. 成功构建重组真核表达质粒 pcDNA3 .;1A DCN;转染MsC并筛选出 2个阳性克隆株。
- Aim To construct the Pichia pastoris expression system of RS gene. 目的建立白藜芦醇合酶基因的毕赤酵母表达系统。
- The eukaryotic expressing vector encoding human cbl is constructed and it expresses flag-cbl fused protein correctly in COS-7 cells. 成功构建了N 末端带flag标签的cbl真核表达载体 ;并使其在真核细胞COS 7中表达 .
- eukaryotic expression system 真核表达系统
- Objective To construct endothelial cell-specific molecular-1(ESM-1) eukaryotic express vector,transfect it into human umbilical vein endothelial cells( HUVECs,ECV304) and express it in vitro . 目的 构建人内皮细胞特异性分子 1(ESM 1)真核表达载体 ,转染人脐静脉内皮细胞系ECV30 4 ,并在ECV30 4中获得表达。
- The chimeric antigen produced by prokaryotic expressing system will be promisingly used to detect and diagnose AIDS in our country. 本论文利用原核表达体系制备的嵌合抗原有望用于我国艾滋病的检测与诊断;
- Results The bicistronic eukaryotic expression plasmid was corrected and can co - express human BMP -2 and VEGF165 mRNA in vitro. 该重组质粒能在体外同时表达BMP2及VEGF165 mRNA。
- Objective:To construct and express a recombinant eukaryotic expression vector bearing fusion gene of human IL-2 cDNA gene and Fc fragment. 目的 :构建含人IL 2cDNA基因和免疫球蛋白Fc片段融合基因的真核表达载体pcDNA 3.;1IL 2 /Fc;并在真核细胞中表达;以期用于乙型肝炎病毒 (HBV)DNA疫苗的研究。